Establishment of Human Neuroblastoma Cell-Line (SK-N-SH) as an In Vitro Model of Morphine Addiction.

Morphine is a schedule II-controlled substance that used to allow the diminution of intra-operative, post-operative or chronic pain. However, its usage limited due addiction and overdose liabilities. was observed cause tolerance, dependence withdrawal in human. Justification: date lack scientific evidence morphine carried out by using specific single human neuroblastoma cell-line (SK-N-SH). Therefore, this study performed establish model cell line. The cells were exposed for 24 hrs before treatment with methadone, as an anti-withdrawal drug subsequence hours. cytosolic fraction different objectives including receptor affinity, properties, endocytic machinery, desensitization internalization cellular adaptation. result shows methadone bind µ-opioid receptor. morphine-treated increase expression markers, have low rate reduce Those changes normalized methadone. As whole, it postulated line, SK-N-SH, can be in-vitro demonstrate animal testing.